USDA/GIPSA Proficiency Program

Testing for the Presence of Biotechnology Events in Corn and Soybeans

February 2003 Sample Distribution

 

Date of Report:  August 22, 2003

 

 

Purpose of USDA/GIPSA Proficiency Program

Through the USDA/GIPSA Proficiency Program, USDA seeks to improve the overall performance of testing for biotechnology-derived grains and oil seeds.  The USDA/GIPSA Proficiency Program helps organizations identify areas of concern and take corrective actions to improve testing capability and reliability.

 

Program Description

In February 2003, USDA/GIPSA’s Technical Services Division expanded the program to offer samples for qualitative or quantitative analysis.  Participants, in response to GIPSA’s request, specifically request samples for either qualitative or quantitative analysis.  GIPSA then mails the participant the samples they requested:  Twelve (12) corn samples and three (3) soybean samples.  The samples for qualitative analyses contain different combinations of biotechnology events at a single concentration.  The samples for quantitative analyses contain combinations of biotechnology events at various concentrations. 

 

Qualitative Sample Analysis.  Samples for qualitative analysis are prepared gravimetrically using non-transgenic corn and biotechnology-derived corn, and the events present in the sample are at approximately 0.1 %.  The participants report the samples as either positive or negative for each of the events. The corn samples contain various combinations of the events T25, CBH351, MON810l, GA21, E176, Bt11 and NK603.   The soybean samples are either non-transgenic soybeans or non-transgenic soybeans fortified with Roundup ReadyTM soybeans at the 0.1 % level.  Each participant receives twelve (12) corn and three (3) soybean samples containing approximately 20 grams of ground grain.

 

Quantitative Sample Analysis.  Samples for quantitative analysis are prepared gravimetrically using non-transgenic corn and biotechnology-derived corn, and the events present in the samples vary in concentration from 0.1 % to 5.0 %.  The corn samples contain various combinations of the events T25, CBH351, MON810l, GA21, E176, Bt11 and NK603.   The soybean samples are either non-transgenic soybeans or non-transgenic soybeans fortified with Roundup ReadyTM soybeans at the 0.1 % or 3.0 % level.  For quantitative sample results, the participants are asked to submit results in weight % of the biotechnology event, or in accordance with their standard reporting procedures.  Each participant receives twelve (12) corn and three (3) soybean samples containing approximately 20 grams of ground grain.

 

Program Participants

GIPSA mailed samples to fifty-eight (58) participating organizations.   Twenty-seven (27) participants requested samples for qualitative analysis, twenty-nine (29) participants requested samples for quantitative analysis, and two participants requested corn samples for qualitative analysis and soybean samples for quantitative analysis.  Participants included organizations from Argentina, Australia, Austria, Brazil, Canada, France, Germany, Ireland, Italy, Japan, Netherlands, Spain, Switzerland, Taiwan, Zimbabwe, and the U.S.  Each participant received a study description, a Chain of Custody form, and a data report form by regular mail and electronic mail.  Participants submitted results by electronic mail, FAX, or regular mail.  No methodologies were specified, and organizations used DNA- and protein-based testing technologies. 

 

Fifty-four (54) of the participating organizations returned results by the deadline date.  Twenty-six (26) participants submitted results for qualitative corn and/or soybean samples, twenty-six (26) participants submitted results for quantitative corn and/or soybean samples, and two participants submitted results for qualitative corn samples and quantitative soybean samples. 

 

Those organizations giving GIPSA permission are listed by name in an attachment to this report (Attachment 1).  Those organizations giving GIPSA permission are also listed by name in the Proficiency Program February 2003 Summary Tables (Tables 1 and 2).  All other organizations were assigned a Participant Identification Number.

 

Data Summary Results

Data submitted by the participants are summarized in this report, including tables and charts.  The data are summarized to reflect the number of participants who submitted results, the number of participants who classified all samples correctly as either positive or negative for the event, and the percentage of samples that were classified correctly by all participants. 

 

Participants were given the option of receiving samples for qualitative or quantitative analysis.  Participants receiving samples for qualitative analysis reported the presence or absence of a particular event in each sample.  Participants receiving samples for quantitative analysis reported the concentration of a particular event in each sample.   Due to the complexity of the data, this report summarizes the data in two sections:

 

Qualitative Data Summaries.  This section includes a summary of the following sample results:

·             Samples for qualitative analysis:  DNA-based results

·             Samples for quantitative analysis:  DNA-based results

·             Samples for qualitative analysis:  Protein-based results

·             Samples for quantitative analysis:  Protein-based results

 

Quantitative Data Summaries.  This section provides summaries of DNA-based quantitative analysis data submitted by participants who received samples for quantitative analysis.  This section presents data only on a composite basis and does not reflect any individual participant data assessment.  The following information is presented:

·             Average analytical results reported for each fortification level for each event (corn and soybean)

·             Coefficients of variation (CVs) for the analytical results reported for each fortification level for each event (corn and soybean)

·             False positive and false negative results reported for the each fortification level for each event (corn and soybeans)

 


Qualitative Data Summaries

Samples for Qualitative analysis:  DNA-Based Results

The data submitted by the participants who received samples for qualitative analysis were assessed on a qualitative basis.  If the event was present in the sample and the participant reported the sample as positive for the event that was considered a correct response.  Conversely, if the event was present in the sample and the participant reported the sample as negative for the event that was considered an incorrect response.  Similarly, if the event was not present in the sample and the participant reported the sample as negative for the event that was considered a correct response.  Conversely, if the event was not present in the sample and the participant reported the sample as positive for the event that was considered an incorrect response.  

 

Table 1 shows results reported by each participating organization on qualitative samples analyzed using DNA-based procedures.  Participants are either listed by name or by a Participant Identification Number. 

 

The data area also summarized on a composite basis for each grain (corn and soybean) event in Graph 1 (Summary Reported Qualitative Sample Analyses for All Organizations) showing the percentage of correct responses and number of sample analytical results submitted for each transgenic event in corn and soybeans.

 

Table 1.  Samples for Qualitative Analysis:  DNA-Based Results by Participant



Graph 1.  Summary Reported Qualitative Sample Analyses for All Organizations


 

Samples for Quantitative analysis:  DNA-Based Results

 

Quantitative data are presented in two ways.  First, the participants were evaluated on their ability to detect the biotech event contained in the quantitative test samples (Table 2 and Graph 2).  Second, the participant results compared on a composite basis and compared to the target level of fortified biotech events (Table 5 and Graph 3).

 

As with the samples for qualitative analysis, if the event was present in the sample and the participant reported the sample as positive for the event that was considered a correct response.  Conversely, if the event was present in the sample and the participant reported the sample as negative for the event that was considered an incorrect response.  Similarly, if the event was not present in the sample and the participant reported the sample as negative for the event that was considered a correct response.  Conversely, if the event was not present in the sample and the participant reported the sample as positive for the event that was considered an incorrect response.  

 

Table 2 shows results reported by each participating organization on quantitative samples analyzed using DNA-based procedures.  Participants are either listed by name or by a Participant Identification Number. 

 

The data are summarized on a composite basis for each grain (corn and soybean) event in Graph 2 (Summary Reported Quantitative Sample Analyses for All Organizations) showing the percentage of correct responses and number of sample analytical results submitted for each transgenic event in corn and soybeans.

 

 

 

 


Table 2.  Samples for Quantitative Analysis:  DNA-based Results by Participants


 

Graph 2.  Summary Reported Quantitative Sample Analyses for All Organizations

 


 

 

 

 


Qualitative Data Summaries

Samples for Qualitative analysis:  Protein-Based Results

 

Table 3 shows results reported by each participating organization on qualitative samples analyzed using protein-based procedures.  This table presents data from both Lateral Flow Strip and ELISA technologies.  Participants are either listed by name or by a Participant Identification Number. 

 

Table 3.  Samples for Qualitative Analysis:  Protein-Based Results by Participant


 

 

Qualitative Data Summaries

Samples for Quantitative analysis:  Protein-Based Results

Table 4 shows results reported on samples for quantitative analyses using protein-based testing technology.  As indicated in the table, no participant submitted results on the analysis of corn samples using the Lateral Flow Strip and one participant submitted results on the analysis of the soybean samples using the Lateral Flow Strip (qualitative results).  One participant submitted quantitative results on the analysis of the corn samples using the ELISA plate assay technology, and two participants submitted quantitative results on the analysis of the soybean samples using the ELISA plate assay technology.
Table 4.  Samples for Quantitative Analysis:  Protein-Based Results by Participant


 

Quantitative Data Summaries

Organizations that requested samples for quantitative analysis received twelve corn samples and three soybean samples.  The twelve corn samples consisted of duplicate samples from six different formulations.  This resulted in a varying number of reported results for each event/concentration combination. 

 

Table 5 summarizes all results reported on a quantitative basis.  As indicated in the table, samples contained various concentrations of the transgenic events:

 

·                    Corn:  0.1 %, 0.5 %, 1.5 %, 3.0 % and/or 5.0 %

·                    Soybean:  0 %, 0.1 % or 3.0 %

 

Note:  It is important to understand that there are no internationally recognized standard reference materials for all transgenic events.   The transgenic seed or grain used to prepare these samples was made available to GIPSA by the Life Science Organizations.  Care was taken to ensure the material was either essentially 100 % positive for the event, or adjusted accordingly.  The fortified samples were prepared using a process that has been verified to produce homogenous mixes, and representative samples were analyzed to ensure proper fortification and homogeneity.

 

The data from Table 5 are presented in Graph 3 (Quantitative Sample Results:  % of Fortification Level Reported for All Organizations) and Graph 4 (Quantitative Sample Results:  Coefficients of Variation).  Table 6 shows the number of false positives reported on samples that did not contain an event, and the number of false negatives reported on samples that contained an event.  The data from Table 6 are presented in Graph 5 (Quantitative Corn/Soy Samples Results:  False Positive/False Negatives for all Organizations).  As can been seen in the table, the false positive rate, i.e., a positive result was reported for an event that the sample did not contain, varied among the events.  The highest percentage of false positives was reported for events T25 (10 %) and Bt11 (12.5 %).  No false positives were reported for CBH351 and NK603, and the false positive rates for MON810, GA21 and E176 were less than 10 %.  One false positive result (4.5 %) was reported for the soybean event.

False negative rates, i.e., a negative result was reported for an event that the sample did contain, ranged from 16.7 % (event Bt11) to 32.8 % (MON810) at the 0.1 % fortification level.  As might be expected, at the higher fortification levels the false negative rate decreased, but it is interesting to note that events T25, MON810 and GA21 still had false negatives reported at the 3.0 % fortification level.  At fortification levels above the 0.1 % no false negatives were reported for events CBH351 and NK603.  One false negative result was reported for the soybean event at the 0.1 % level, and one false negative result was reported for the soybean event at the 3.0 % level.

 

General observations from these data:

 

1.                  The means for corn events T25, GA21, Bt11 and NK603 were generally within + 25 % of the fortification levels. 

 

2.                  The means for CBH351, MON810 and E176 were consistently lower that the fortification level. 

 

3.                  The means for the soybean event were consistently higher than the fortification level, especially at the 0.1 % fortification level.

 

4.                  As might be expected, the Coefficients of Variation (CV) decreased as the fortification level increased.  At the 0.1 % fortification level the CV’s ranged from 64.8 % to 153.1 % for the corn events.  At the 3.0 % fortification level, the CV’s were lower, ranging from 40.1 % to 52.9 %.  For the soybean event, the CV’s ranged from 220.7 % at the 0.1 % fortification level to 31.4 % at the 3.0 % fortification level.

 


Table 5.  Summary Quantitative Sample Analyses

 

Fortification                                                                 Standard                   Results

Level                Mean                Range                       Deviation CV            Reported

Event T25 (Corn)

0 %                   0 %                   NA                                     NA           NA           40    

0.1 %                0.15 %              0.00 % - 0.60 %                 0.14          94.6 %      60

0.5 %                0.55 %              0.00 % - 1.10 %                 0.35          63.5 %      28

1.5 %                1.65 %              0.00 % - 4.20 %                 1.06          64.3 %      48

3.0 %                2.27 %              0.00 % - 3.80 %                 1.20          52.9 %      12

Event CBH351 (Corn)

0 %                   0 %                   NA                                     NA           NA           30

0.1 %                0.07 %              0.00 % - 0.20 %                 0.05          71.5 %      18

0.5 %                0.33 %              0.01 % - 1.10 %                 0.24          71.7 %      32

1.5 %                1.20 %              0.40 % - 3.00 %                 1.25          103.8 %    6

3.0 %                1.85 %              0.01 % - 3.00 %                 0.97          52.5 %      18

Event MON810 (Corn)

0 %                   0.04 %              0.00 % - 0.82 %                 0.17          390.4 %    42    

0.1 %                0.12 %              0.00 % - 0.50 %                 0.18          153.1 %    59

0.5 %                0.29 %              0.00 % - 0.45 %                 0.14          49.0 %      13

1.5 %                1.41 %              0.00 % - 4.10 %                 1.24          88.1 %      36    

3.0 %                1.75 %              0.00 % - 5.00 %                 1.03          58.7 %      37

5.0 %                3.04 %              0.58 % - 5.00 %                 1.18          39.0 %      36

Event GA21 (Corn)

0 %                   0.01 %              0.00 % - 0.20 %                 0.03          543.6 %    54

0.1 %                0.14 %              0.00 % - 0.35 %                 0.12          92.5 %      10

0.5 %                0.52 %              0/00 % - 1.82 %                 0.37          71.5 %      26

1.5 %                1.74 %              0.80 % - 5.03 %                 0.88          50.6 %      52

3.0 %                2.32 %              0/00 % - 3.12 %                 0.82          35.2 %      24

Event 176 (Corn)

0.0%                          0.17 %              0.00 % - 2.70 %                 0.68          400.0 %    16            

0.1%                          0.08 %              0.00 % - 0.50 %                 0.09          121.3 %    34

0.5 %                0.31 %              0.00 % - 1.90 %                 0.26          90.3 %      68

1.5 %                0.96 %              0.00 % - 2.50 %                 0.51          53.5 %      34

3.0 %                1.80 %              0.48 % - 5.00 %                 0.84          46.7 %      34

5.0 %                3.01 %              1.00 % - 5.00 %                 1.12          37.3 %      24

Event Bt11 (Corn)

0 %                   0.03 %              0.00 % - 0.50 %                 0.09          347.9 %    40

0.1 %                0.10 %              0.00 % - 0.20 %                 0.06          64.8 %      12

0.5 %                0.56 %              0.00 % - 2.00 %                 0.36          64.7 %      55

1.5 %                1.36 %              0.00 % - 3.30 %                 0.64          47.4 %      67

3.0 %                2.43 %              0.70 % - 5.00 %                 0.97          40.1 %      32

Event NK603 (Corn)

0 %                   0 %                   NA                                     NA           NA           9

0.1 %                0.15 %              0.00 % - 0.50 %                 0.15          96.9 %      20

0.5 %                0.43 %              0.15 % - 1.40 %                 0.27          62.4 %      40

1.5 %                1.14 %              0.50 % - 2.20 %                 0.51          44.7 %      32

3.0 %                2.60 %              0.90 % - 4.80 %                 1.21          46.7 %      20

Roundup ReadyTM (Soybean)

0 %                   0 %                   NA                                     NA           NA           18

0.1 %                0.31 %              0 % – 3.17 %                     0.68          220.7 %    20

3.0 %                3.71 %              2.2 % - 5.0 %                     1.17          31.4 %      20


Graph 3.  Quantitative Samples Results:  Percentage of Fortification Level


 

 

Graph 4.  Quantitative Sample Results:  Coefficients of Variation


 

 
Table 6.  False Positive (% False P.) and False Negative (% False N.):  Corn and Soybeans

                                           CBH         MON

Level                         T25           351            810            GA21       E176         Bt11          NK603     RR Soy

0 %                                                                                                       

False  Pos.         4               0               3               4               1               5               0               1

# of Obs.           40             30             42             54             16             40             8               22

% False Pos.      10 %                 0 %           7.1 %        7.4 %        6.3 %        12.5 %      0 %           4.5 %

0.1  %

False Neg. 13             4               20             3               12             2               4               1

# of Obs.           60             18             61             10             34             12             20             23

% False Neg.     21.7 %      22.2 %      32.8 %      30.0 %      35.3 %      16.7 %      20.0 %      4.3 %

0.5  %

False Neg. 2               0               2               2               6               3               0               N/A*

# of Obs.           28             32             13             26             68             55             40            

% False Neg.     7.1 %        0 %           15.4 %      7.7 %        8.8 %        5.5 %        0 %          

1.5  %

False Neg. 2               0               2               0               0               3               0               N/A

# of Obs.           48             6               36             52             34             67             32

% False Neg.     4.2 %        0 %           5.6 %        0 %           0 %           4.5 %        0 %

3.0  %                                

False Neg. 1               0               2               2               0               0               0               1

# of Obs.           12             20             37             26             34             32             20             23

% False Neg.     8.3 %        0 %           5.4 %        7.7 %        0 %           0 %           0 %           4.3 %

5.0 %      

False Neg.         N/A          N/A          0               N/A          0               N/A          N/A          N/A

# of Obs.                                             36                              24

% False Neg.                                       0 %                            0 %

*N/A means no samples were analyzed at this level of fortification

 

 Graph 5.  False Positive/False Negative Results:  Corn and Soybeans



USDA/GIPSA Proficiency Program for February 2003:  General Conclusions

Organizations testing for transgenic events do a good job of identifying the presence or absence of transgenic events down to levels of 0.1 %.  The results obtained on the samples for qualitative analysis were similar to the results obtained previously.   

 

This was the first time the USDA/GIPSA Proficiency Program offered participants the opportunity to analyze samples that contained various amounts of the transgenic events and report quantitative results.  Approximately half of the participants chose samples for qualitative analysis, and the other half chose samples for quantitative analysis.  The samples for qualitative analysis were fortified at 0.1 %, and the samples for quantitative analysis were fortified at levels ranging from 0.1 % to 5.0 %. 

 

With respect to quantitative analysis, the variability of the results was relatively high, especially at lower fortification levels, but given the lack of official methods and internationally recognized reference materials, this was not an unexpected observation. Generally, the mean values reported were lower than the target fortification levels, especially at the higher fortification levels.  Since this was the first time samples were offered for quantitative analysis, the quantitative results were summarized in a table rather than by individual organization.  In the future results will be provided for those organizations that report quantitative results on an individual participant basis.

 

May 2003 Sample Distribution

Samples were distributed in May 2003, and the next distribution is scheduled for August 2003.   Participants were again given the opportunity to receive samples for qualitative or quantitative analysis.  In addition, two new transgenic events have been deregulated in the U.S. and these events will likely be added to the program in November 2003.  

 

To obtain additional information on the USDA/GIPSA Proficiency Program, contact Donald C. Kendall, Biotechnology Program Manager, at US 816-891-0463, or by e-mail at biotech-lab@usda.gov.

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

Attachment 1.  GIPSA Proficiency Program Participants:  February 2003

 

The following organizations gave GIPSA permission to identify them as participants in the GIPSA Proficiency Program:

 

Company          A. Bio. C – Molecular Biology Division

Address             2, rue Jacques Monod        

                          F-33651 MARTILLAC

                          France

Contact              Dr. F. Bois

Telephone 33 5 56 64 03 55

Fax                    33 5 56 64 07 66

E-mail                kaligene@wanadoo.fr

 

Company          Advanta Seeds B.V.

Address             VanderHaveweg 2

                          4411 RB Rilland

                          The Netherlands

Contact              Jan Pertijs

Telephone +31(0) 113 557100

Fax                    +31(0) 113 552237

E-mail                jan.pertijs@nl.advantaseeds.com

 

Company          AgriQuality GMO Services

Address             Suite 19, TEC   

                          2 Park Drive

                          Bundoora VIC  3083

                          Australia

Contact              Megan Smithwick, Ph.D.

Telephone 613 9479 5068

Fax                    613 9479 5693

E-mail                smithwickm@agriquality.com.au

 

Company          AINIA (Instituto Tecnologico Agroalimentario)      

Address             Benjamin Franklin 5-11

                          Parque Tecnologico

                          46980 Paterna

                          Valencia

                          Spain

Contact              David Tomas

Telephone +34961366090

Fax                    +34961318008

E-mail                dtomas@ainia.es

 

Company          Bayerisches Landesamt fur Gesundheit und Lebensmittelsicherheit (LGL)

Address             Veterinarstsr. 2

                          D-85764 Oberschleissheim

                          Germany

Contact              Sven Pecoraro, Ph.D.

Telephone +49-89-31560-585

Fax                    +49-89-31560-458

E-mail                Sven.Pecoraro@lgl.Bayern.de

Attachment 1.  GIPSA Proficiency Program Participants:  February 2003

 

Company          Behoerde fur Umwelt und Gesundheit

Address             Institut fur Hygiene und Umwelt

                          Marckmannstr.129b, Haus 6

                          D20539 Hamburg

                          Germany

Contact              Norbert Hess, Ph.D.

Telephone +49 40 42845 3629

Fax                    +49 40 42845 3840

E-mail                norbert.hess@bug.hamburg.de

 

Company          Biogenetic Services, Inc.

Address             801 32nd Avenue

                          Brookings, SD  57006

Contact              Alex Kahler

Telephone 800-423-4263/605-697-8500

Fax                    605-697-8507

E-mail                biogene@brookings.net

 

Company          Bolsa de Comercio de Rosario        

Address:            Córdoba 1402

Rosario S2000AWV

Argentina

Contact              Juan J. Giorda 

Telephone          54-341-4211000

Fax                    54-341-4213471  ext 2211

E-mail                jjgiorda@bcr.com.ar  

 

Company          California Seed and Plant Lab

Address             7877 Pleasant Grove Road

                          Elverta, CA  95626

Contact              Parm Randhawa

Telephone 916-655-1581

Fax                    916-655-1582

E-mail                randhawa@calspl.com

 

Company          Chemisches Landes- und Staatliches Veterinaruntersuchungsamt

Address             Von-Esmarch-Str.12 

                          D-48149 Muenster    

                          Germany

Contact              Claudia Bruenen-Nieweler, Ph.D.

Telephone 49-251-9821-186

Fax                    49-251-9821-250

E-mail                nieweler@cvua.nrw.de

 


Attachment 1.  GIPSA Proficiency Program Participants:  February 2003

 

Company          CTNCV-Laboratorio del Ebro

Address             Calle Santa Gema 56

                          31570 San Adrian

                          Navarra

                          Spain

Contact              Blanca Jauregui, Ph.D.

Telephone 34 948 670159

Fax                    34 948 696127

E-mail                bjauregui@ctncv.es

 

Company          Eurofins Scientific, Woodson-Tenent Laboratories Division

Address             3507 Delaware

                          Des Moines, IA  50313

Contact              David Pinero

Telephone 515-265-1461

Fax                    515-266-5453

E-mail                davidpinero@eurofinsus.com

 

Company          European Commission - JRC

Address             Via Fermi 1

I-21020 Ispra (VA)

Italy

Contact              Marco Mazzara

Telephone 0039 0332 785773

Fax                    0039 0332 789333

E-mail                marco.mazzaro@jrc.it

 

Company          FASMAC CO., LTD

Address             5-1-3 Midorigaoka, Atsugi-shi

                          Kanagawa 243-0041 

JAPAN

Contact              Dr. Satoshi Futo

Telephone +81 46-295-8787

Fax                    +81 46-294-3738

E-mail                sfuto@fasmac.co.jp

 

Company          Federal Biological Research Centre for Agriculture and Forestry

Address             Institute for Plant Viology, Microbiology and Biosafety

                          Messeweg 11-12

                          D-38104 Braunschweig

                          Germany

Contact              Dr. Antje Dietz-Pfeilstetter

Telephone 0531-2993819

Fax                    0531-2993013

E-mail                a.dietz@bba.de

 


Attachment 1.  GIPSA Proficiency Program Participants:  February 2003

 

Company          Federal Institute for Risk Assessment BfR

Address             Thielallee 88-92

                          D-14195 Berlin

                          Germany

Contact              Jutta Zagon, Ph.D.

Telephone 49-30-8412-3876

Fax                    49-30-88412-3685

E-mail                j.zagon@bfr.bund.de

 

Company          GeneScan Analytics GmbH, Freiburg

Address             Engesserstr. 4

79108 Freiburg i. Br.

                          Germany

Contact              Dr. Castor Menendez

Telephone +49-(0)761-5038

Fax                    +49-(0)761-5038-111

E-mail                gmoanalytics@genescan.com

 

Company          GeneScan USA, Inc.        

Address             101 Woodland Highway

                          Belle Chasse, LA  70037

Contact              Frank Spiegelhalter

Telephone 504-398-0940

Fax                    504-398-0945  

E-mail                fspiegel@gmotesting.com

 

Company          Genetic ID NA, Inc.

Address             501 Dimick Drive

                          Fairfield, Iowa  52557        

Contact              Jane Pappin      

Telephone 641-472-9979, ext. 122                     

Fax                    641-470-1504  

E-mail                jpappin@genetic-id.com

 

Company          Institute of Food Chemistry and Technology

Address             Graz, University of Technology   

                          Petersgasse 12/2

                          8010 Graz

                          Austria

Contact              Ursula Muelleder, Ph.D.

Telephone 0043 316 873 6499

Fax                    0043 316 873 6971

E-mail                ursula.muelleder@tugraz.at

 


Attachment 1.  GIPSA Proficiency Program Participants:  February 2003

 

Company          IRTAGen Servei d'Anŕlisis Genčtiques

Address             Crta. Cabrils, s/n

                          08348-CABRILS

                          Barcelona

                          Spain

Contact              Imma Folch

Telephone 93 750 75 11

Fax                    93 753 39 54

E-mail                imma.folch@irta.es

 

Company          Landesamt fur Verbraucherschutz und Landwirtschaft   

Address             Referat 5.11 (Biologische Methoden)  

                          Pappelallee 20

14469 Potsdam

Germany

Contact              Dr. Holgen Pilsl

Telephone 0335 52 17 127

Fax                    0335 52 17 205

E-mail                Holger.Pilsl@LVL.Brandenburg.de

 

Company        Landesuntersuchungsanstalt fur das Gesundheits-und Veterinarwesen

Sachsen

Address             Sitz Dresden     

                          Amtliche Lebensmitteluberwachung     

                          Fachgebiet 6.6

Postfach 2002744

D – 01192 Dresden

Germany

Contact              Dr. Gerda Hempel

Telephone +49-0351-8144-474

Fax                    +49-0351-8144-497

E-mail                gerda.hempel@lua.sms.sachsen.de

 

Company          LAV Sachsen-Anhalt

Address             Freiimfelder Str. 66/68

                          D-061112 Halle

                          Germany

Contact              Dr. Dietrich Maede

Telephone +49 345 5643 222

Fax                    +49 345 5643 439

E-mail                maede@lvluahal.ml.lsa-net.de

 

Company          Monsanto

Address             700 Chesterfield Parkway North

                          Mail Zone:  BB5k

                          St. Louis, MO  63189

Contact              Ming Y. Deng, Ph.D.

Phone                (636) 737-5854

Fax                    (636) 737-6134

E-mail                ming.y.deng@monsanto.com

Attachment 1.  GIPSA Proficiency Program Participants:  February 2003

 

Company          National Institute of Biology

Address             Vecna pot 111

                          1000 Ljubljana

                          Slovenia

Contact              Dr. Jana Zel

Telephone +386 1 4233388

Fax                    +386 1 2573 847

E-Mail               jana.zel@nib.si

 

Company          National Laboratory of Foods and Drugs, Department of Health, Taiwan

Address             161-2, kuen Yang Street

Nankang   

Taipei, Taiwan   

Contact              Dr. Lih-Ching Chiueh

Telephone          02-26531273

Fax                    02-26531268

E-mail                clc1025@nlfd.gov.tw

 

Company          Nippon Yuryo Kentei Kyokai Yokohama Laboratory

              (Japan Oil Stuff Inspectors Corporation)

Address             Bankokubashi Bldg 5-26-1

                          Kaigan-dori Naka-ku

                          231-0002,Yokohama

Japan

Contact              Ms Kumi Goto

Phone                045-641-1037

Fax                    045-641-1038

E-mail                goto@nykk.or.jp

 

Company          OMIC USA Inc.

Address             Laboratory and Inspection

                          1200 NW Naito Parkway

                          Suite 100

                          Portland, OR  97209

Contact              Farin Hajar, DNA Laboratory Manager

Telephone 503-224-5929

Fax                    503-223-9436

E-mail                dna.us@omicnet.com

 

Company          SGS do Brasil Ltda.

Address             Av. Vereador Alfredo das Neves, 480         

                          Alemoa

                          11095-510

Santos-SP

                          Brazil

Contact              Erica Lui Reinhardt

Phone                55 13 3295-9558

Fax                    55 13 3295-9553

E-mail                erica_reinhardt@sgs.com

 


Attachment 1.  GIPSA Proficiency Program Participants:  February 2003

 

Company          Silliker Laboratories of Iowa

Address             405 8th Ave SE

                          Cedar Rapids, IA  52401

Contact              Dan Wetsch      

Telephone 319-366-3570

Fax                    319-366-4018  

E-mail                daniel.wetsch@silliker.com

                

Company          Sistemas Genomicos S. L.

Address             Valencia Technology Park

                          Benjamin Franklin Avenue 12

                          46980 Paterna (Valencia)

                          Spain, Europe

Contact              Angela Perez     

Telephone +34-96-199-4266

Fax                    +34-96-199-4265

E-mail                info@sistemasgenomicos.com

 

Company          Staatliches Veterinaeruntersuchungsamt

Address             Zur Taubeneiche 10-12

                          D-59821 Arnsberg

                          Germany

Contact              Jochen Kilwinski, Ph.D.

Telephone 49-2931-809-220

Fax                    49-2031-809-290

E-mail                kilwinski@svia-arnsberg.nrw.de

 

Company          Staatliches Veterinaeruntersuchungsamt Detmold

Address             Westerfeldstrasse l

                          D-32758 Detmold

                          Germany

Contact              Dr. Margit Hagen

Telephone +49 (0)5231/911-740

Fax                    +49 (0)5231/911-503

E-mail                margit.hagen@svua-detmold.nrw.de

 

Company          Staatliches Veterinaeruntersuchungsamt Krefeld           

Address             Deutscher Ring 100

                          D-47798 Krefeld

                          Germany

Contact              Dr. Heike Naumann

Telephone 0049-2151-849287

Fax                    0049-2151-849110

E-mail                naumann@svua-krefeld.nrw.de

 


Attachment 1.  GIPSA Proficiency Program Participants:  February 2003

 

Company          State Laboratory

Address             Abbotstown

                          Dublin 15

                          Ireland      

Contact              Patricia Bonner

Telephone +353 1 8025871

Fax                    +353 1 8217320

Email                 pbonner@statelab.ie

 

Company          Syngenta Seeds Ltda

Address             Molecular Genetics Lab

                          BR452 Km 142

                          Uberlandia-MG

                          Brazil

                          38405-232

Contact              Cristhiane Abegg Bothona

Phone                55-34-32334510

FAX                  55-34-32166537

E-mail                cristhiane.bothona@syngenta.com

 

Company          Thuringer Landesamt fur Lebensmittelsicherheit und Verbraucherschutz

Address             Sitz Jena

                          Amtliche Lebensmitteluberwachung

                          Nauburger Str. 96 b

                          D-07743 Jena

                          Germany

Contact              Annelis-Reanate Winterstein

Telephone 0049-3641-486-201

Fax                    0049-3641-486-114

E-mail                awinterstein@tllv.thueringen.de